Skip to main content

Long-term efficacy of ultrasound-guided high-intensity focused ultrasound treatment of breast fibroadenoma



To assess the long term efficacy and tolerability of one or two ultrasound (US)-guided high-intensity focused ultrasound (HIFU) treatment in patients with breast fibroadenoma (FA).


Twenty patients with 26 FA were selected for US-guided HIFU. The therapy was performed in one or two sessions. FA volume was assessed before and followed up to 24 months after the last HIFU. After each treatment, adverse events were evaluated.


In 19/26 FA (73.1%) one HIFU was performed (group 1), whereas 7/26 FA (26.9%) received second HIFU (group 2) 6-9 months (median, 7 months) after the first session. In group 1 and 2, FA volume decreased significantly at 1-month (p < 0.001) and 3-month follow-up (p = 0.005), respectively, and continued to reduce until 24-month follow-up (p < 0.001 and p = 0.003, respectively). At 24 months, mean volume reduction was 77.32% in group 1 and 90.47% in group 2 (p = 0.025). Mild subcutaneous edema was observed in 4 patients and skin erythema in 3 patients.


US-guided HIFU represents a promising non-invasive method with sustainable FA volume reduction and patient’s tolerability. Although one treatment is highly efficient, the volume reduction can be increased with second treatment.

Trial registration

NCT01331954. Registered 07 April 2011.


Breast fibroadenomas (FA) are the most prevalent benign tumours, accounting for up to 70% of benign breast lesions [1, 2]. Most frequently, they affect females in the reproductive period with two peaks of incidence in the third and in the fifth decade of life. They may also occur after menopause as a result of hormone replacement therapy [3, 4]. Although FA, typically consisting of stromal and epithelial cells, usually occurs unilaterally, multiple lesions in the same breast or bilaterally may arise in 20% of cases [4, 5]. Most patients clinically present with palpable breast lump, detected during medical examination or self-examination, or occasionally with breast pain [2, 6]. Without treatment, a minority of FA decrease in size or disappear, more than half of them remain unchanged, and some of them significantly increase [7]. The long-term risk for breast carcinoma in women with FA has not been established [8, 9] and therefore, a conservative approach to the treatment seems to be safe especially in younger patients [5, 10, 11]. However, some patients prefer elimination due to large size, physical discomfort and anxiety about growth or malignancy. The widely employed surgical excision may result in scar formation, breast volume loss and potential for nipple areolar distortion or displacement [12]. Different non-surgical techniques have been reported. Vacuum-assisted biopsy and cryoablation both demonstrated excellent efficacy, safety and high level of patients’ satisfaction though being minimally invasive [1315]. The thermoablation procedures such as laser, radiofrequency or microwave ablation have been reported, but they are more frequently applied in breast cancer patients [1619].

The only non-surgical and non-invasive procedure, employed until today, is high-intensity focused ultrasound (HIFU), where thermal destruction is achieved by precisely delivered energy to a given area in soft tissue without interrupting skin integrity. Although this technique has been applied in the treatment of breast cancer, prostate tumours, uterine fibroids, liver or renal tumours, the experience in breast FA is limited [20]. More than a decade ago, magnetic resonance (MR)-guided HIFU ablation of FA was proposed on the basis of demonstrated feasibility and efficacy in a small group of patients [21]. Recently, Peek et al. showed 43.5% mean FA volume reduction 6 months after circumferential ultrasound (US)-guided HIFU treatment of 19 patients [22]. A multicentre study of Kovatcheva et al. established that US-guided HIFU treatment of 51 FA resulted in 72.5% volume reduction at 1-year follow-up [23]. However, data on prolonged effectiveness and persistence of volume reduction are missing.

In this prospective study, our aim was to assess the long-term treatment efficacy and tolerability of one or two US-guided HIFU sessions in patients with breast FA.


Study design

In our single-centre study, performed between May 2011 and April 2015, from 58 females with clinical suspicion of one or more FA in one or both breasts, 20 symptomatic patients with 26 FA were selected for treatment with US-guided HIFU. The study was approved by the local ethics committee and informed consent was obtained from all individual participants included in the study.

The eligibility criteria included the age of 18 years or more; the clinical diagnosis of breast FA based on palpation, US examination alone for patients < 35 years of age, and mammography in addition for women older than 35 years with Breast Imaging and Reporting Data System (BI-RADS) score ≤2; and a histological confirmation after large-core biopsy using a 16-gauge needle size performed at least two weeks before therapy and verified by two independent readers. The targeted FA size had to be larger than 10 mm, without macrocalcifications inducing a substantial shadowing and situated within the treatable area, which was 5 to 28 mm from the skin surface. The intended size of the ablation unit was 9 mm in length and 1.8–2.5 mm in width and the depth of each ablation unit was adjusted automatically to be centred with the antero-posterior diameter of the target. The rib cage had to be outside the treatment cone or at least 10 mm behind the focal point. Those criteria had to be fulfilled in treatment conditions, once the breast was immobilized and eventually compressed. We excluded women who were pregnant or lactating, those with US suspicions for malignancy, BI-RADS score > 2 or with microcalcifications within the lesion. Also those with history of breast cancer, history of laser or radiation therapy of the targeted breast and those with breast implants were excluded. Thirty-three patients did not meet the inclusion criteria for the following reasons: pregnancy (n = 1); unconfirmed diagnosis of FA (n = 11); FA inaccessibility (n = 21) such as localization partly behind the nipple, close to the rib cage, outside the treatable area, macrocalcifications in the lump; 4 patients refused to participate.

At a selection visit prior to HIFU ablation, body mass index (BMI) was calculated according to the formula: weight/height 2 (kg/m2) and US characteristics of FA were evaluated. After the first HIFU, follow-up visits with US assessment of FA were performed at 1, 3 and 6 months. At 6-month follow-up, if FA volume reduced for less than 50% or its absolute volume exceeded 1.5 ml, a second HIFU ablation was performed and all patients were followed-up at 12 and 24 months after the last treatment. Comparison of FA characteristics was conducted on the basis of the number of treatment procedures.

US evaluation

US evaluation of the targeted FA was performed using an 8-MHz linear probe and a real-time color Doppler US system (Aloka, Prosound Alpha 7, Tokyo, Japan). The first dimension (d1) was recorded parallel to the skin in radial position to the nipple. The second dimension (d2) was measured orthogonal to d1, and the third dimension (d3) was measured in anteroposterior direction. The FA volume was calculated by the ellipsoid model (d1 · d2 · d3 · π/6). Volume reduction (%) was calculated as: ([basal volume – final volume] · 100)/basal volume. Before the treatment, the intranodular color flow Doppler (CFD) pattern was evaluated as follows: pattern 0, absence of flow; pattern I, presence of flow with patchy, uneven distribution; pattern II, clearly increased flow with patchy distribution; pattern III, marked increase in blood flow with diffuse homogeneous distribution [24]. US-guided large-core biopsy was carried out in each patient and histological analysis was performed by two independent readers to prove the benign nature of the targeted FA.

HIFU intervention

HIFU treatment was conducted with a real-time US-guided HIFU system (EchoPulse, Theraclion, Paris, France), described in details previously [23]. The machine consists of a corpus with energy generator, a treatment head and a touch screen interface enabling a procedure planning and monitoring. The treatment head consists of an integrated 7.5 MHz linear array transducer for imaging, a 3 MHz spherical transducer (diameter of 38 or 56 mm and focal region of 0.5x1.0x2.5 mm) to generate HIFU and a cooling system to prevent skin burn.

The treatment was performed on an outpatient basis by a single physician (R.K.) with 7 years practice in US-guided HIFU therapy. The patient was placed in a lateral position to reduce the respiratory movements and the breast was supported by a special immobilization system (SenoPad, Theraclion, Paris, France), consisting in an adaptable plate with a silicon pad, a pressure plate and 2 articulated arms designed to fix the breast during the procedure (Fig. 1). Conscious intravenous analgesia was administered. The physician positioned the treatment head facing the targeted part of the breast and outlined the FA in two axes on the touch screen interface. Once the planning was finished, the treatment started with consecutive repeated HIFU pulses and cooling pauses with duration of 6 s and 54 s, respectively. The acoustic output power was 60 W and the intensity was 25 000 W/cm2. The treatment head moved automatically to cover the whole FA volume. The physician controlled the procedure and adjusted the applied energy to obtain the desired tissue reaction, characterized by hyperechoic marks as a sign of tissue damage [25]. When hyperechoic marks did not occur at maximal admissible energy, the treatment was continued at the lower energy level to avoid thermal damage of surrounding tissues. Treatment duration consisted of sonication, cooling and repositioning. During therapy, the patient’s vital parameters were monitored. In case of intolerant pain patients were asked to make a sign, in order to receive additional analgesics and the pause before the next pulse was extended with 30 to 60 s.

Fig. 1
figure 1

Breast support and immobilization system SenoPad (Theraclion, Paris, France)

At the end of the HIFU procedure, pain related to the treatment was subjectively rated using a 0–100 mm visual analogue scale (VAS) and adverse events were assessed. After the 6-month follow-up, the patients completed a satisfaction questionnaire evaluating symptoms and cosmetic improvement with a 4-grade scale (1 – no, 2 – low, 3 – high, 4 – complete satisfaction).

Data analysis

Data analysis was carried out using the Statistica software version 7.1 (StatSoft, Tulsa, OK, USA) and p < 0.05 was considered significant. Data are presented as mean ± standard deviation (SD) or median and range, as appropriate according to the distribution. To compare two groups, we applied the Student’s two-tailed t-test or Mann-Whitney U-test for continuous variables and the Chi-square for categorical variables. Comparisons of multiple variables were performed using ANOVA test, whereas for the follow-up comparison of longitudinally recorded data repeated measures ANOVA test was used. Correlations were calculated with the Pearson correlation test.


Patient and FA characteristics

The mean age of 20 patients was 29.0 ± 10.2 years, 7/20 (35%) were previously operated for FA of the same breast and 7/20 (35%) had more than one FA. Nineteen of 26 FA (73.1%) were treated with one HIFU session (group 1). In 7/26 FA (26.9%) second HIFU ablation was performed (group 2) between 6 and 9 months (median 7 months) after the first session. FA in the group 2 were significantly larger at baseline, otherwise no significant differences in FA characteristics were observed between the two groups (Table 1).

Table 1 Baseline features of breast fibroadenomas treated with 1 session (group 1) or with 2 sessions (group 2) of HIFU

During the first HIFU session, the treated volume, total applied energy, treatment duration and the number of treated sites were significantly higher in group 2 than in group 1. The percentage of the treated volume was significantly lower in group 2, whereas the energy per treated volume and the percentage of hyperechoic marks did not differ between both groups (Table 2). During the second HIFU session, the treated volume and total applied energy in group 2 were still larger compared with the baseline values in group 1, whereas other parameters did not differ significantly (Table 2).

Table 2 Treatment characteristics at each HIFU session

FA volume reduction

In group 1, FA volume decreased significantly as soon as at 1-month follow-up (median 1.44 mL, range 0.21–5.18 mL, p < 0.001 compared with the initial value), and continued to reduce until the 24-month visit (median 0.35 mL, range 0.06–1.21 mL; p < 0.001 compared with the initial value) (Fig. 2). Illustration of FA treated with one HIFU session is shown in Fig. 3. In group 2, a significant FA shrinkage was observed at 3-month follow-up after the first HIFU session (median 4.70 mL, range 0.88–8.02 mL, p = 0.005 compared with the initial value). After the second HIFU session, the significant volume reduction continued until the 24-month visit (median 0.21 mL, range 0.09–1.66 mL, p = 0.003 compared with the initial value) (Fig. 4). Reduction of FA treated with two HIFU sessions is demonstrated in Fig. 5.

Fig. 2
figure 2

Fibroadenoma volume reduction in patients treated with one HIFU session. *p < 0.001 compared with the initial value before treatment (repeated measures analysis of variance test)

Fig. 3
figure 3

Radial scan of left breast fibroadenoma in 27-years old woman treated with one HIFU session. a baseline US shows an oval-shaped hypoechoic well-defined lesion of 1.87 ml of volume; b 6 months after the treatment 64.6% of volume reduction was found; c at 12-month follow-up the volume reduction was 73%; d the tendency continued up to 24 months with 78.6% of total volume reduction

Fig. 4
figure 4

Fibroadenoma volume reduction in patients treated with two HIFU sessions. *p < 0.01 compared with the initial value before treatment (repeated measures analysis of variance test)

Fig. 5
figure 5

Anti-radial scan of right breast fibroadenoma in 39-years old woman treated with two HIFU sessions. a baseline US shows an oval-shaped hypoechoic well-defined lesion with volume of 10.87 ml; b 6 months after the first HIFU ablation 58.2% of volume reduction was observed, but the FA was still large (4.35 ml); c 6 months after the second HIFU ablation the volume reduction was 96.5% from baseline; d the reduction progressed at 12 months up to 98.7%; e at 24-month follow-up the total volume reduction was 99.1%

The percentage of volume reduction was comparable between both groups until 6-month follow-up after the first HIFU (mean 58.04 ± 16.9% in group 1 and 50.44 ± 10.38% in group 2, respectively). However, at 12-month visit after the final treatment the percentage of volume reduction was significantly higher in group 2 that received two treatments (mean 71.02 ± 10.39% in group 1 compared with 86.28 ± 7.64% in group 2, p = 0.002). Similar difference was found at 24-month visit (mean 77.32 ± 13.47% in group 1 compared with 90.47 ± 7.13% in group 2, p = 0.025), when the maximal volume reduction in group 1 and 2 reached 94.67% and 99.13%, respectively. During 24-month follow-up no case of regrowth was observed.

In order to estimate the effects of HIFU on FA shrinkage, we further compared the reduction of individual diameters at 6-month follow-up after the first HIFU. In all FA, the percentages of d1, d2 and d3 reduction were comparable (mean 26.44% ± 12.64%, 25.92 ± 11.44%, 19.99 ± 24.22%, respectively). After HIFU therapy, color Doppler flow decreased or totally disappeared. We found a significant positive correlation of initial CFD pattern with FA volume reduction at 12 months (r = 0.450, p = 0.021).

Safety, tolerability and satisfaction of patients

HIFU was well tolerated by all patients and no serious adverse events were observed. The VAS score at the first HIFU session (mean 40.7 ± 24.6, range 5–78) did not differ significantly from the VAS score at the second session (mean 34.9 ± 17.9, range 7–64). There was no significant correlation between the VAS score and age, BMI, FA depth, total applied energy as well as the applied energy per volume, initial FA volume or the duration of treatment. Up to one week after the first treatment, 9/20 patients (45.0%) reported about mild to moderate pain or tenderness of the treated FA, and similar sensation was reported in 4/7 (57.1%) patients after the second session. No patient needed additional analgesic drugs after the therapy.

During the first HIFU treatment, 4 patients developed mild subcutaneous oedema that disappeared at 1 week without therapy. Immediately after the treatment, mild to moderate erythema was detected in 3 patients treated twice. In 2 of them, this reaction disappeared up to 1 week after each HIFU session. Only in one patient with BMI 17.4 kg/m2 it evoluted as first-degree skin burn with crusts and hyperpigmentation visible up to 6-month follow-up. All side effects were transient and showed complete resolution.

Patients' satisfaction

All patients completed a questionnaire. The level of satisfaction with symptom disappearance was high (grade 3) in 50%, 45% were satisfied completely (grade 4), whereas in 1 patient (5%) the level of satisfaction was low (grade 2). With respect to cosmetic results, 19 out of 20 patients (95%) were satisfied completely (grade 4) and in 1 patient (5%) the level of satisfaction was high.


Our single-center study demonstrates that US-guided HIFU treatment is effective in treating breast FA as indicated by a significant and stable volume reduction at two years follow-up, when the mean reduction was 77.32% after one HIFU session and 90.47% after two HIFU sessions. The procedure is safe, with favourable cosmetic outcome and patient’s satisfaction.

The significant reduction of FA volume as a result of US-guided HIFU is consistent with a report based on multicentre study, where 72.5% FA volume reduction was shown at 1-year follow-up [23]. A favourable treatment effect of HIFU was previously described in patients with breast cancer, in whom coagulation necrosis has been obtained in 78% to 100% of treated breast tumour volume [26, 27]. Based on our data, HIFU ablation results in substantial FA volume reduction 1 month after therapy, and the volume continues to regress during the long-term follow-up. According to this stable tendency, further reduction may be expected. Besides, our data show that with second treatment the volume reduction can be additionally enhanced.

Feasibility and efficacy of HIFU performed under MR guidance were first demonstrated in breast FA more than a decade ago [21]. Compared with MR guidance, US-guided FA treatment is faster, more comfortable for the patient and it does not need contrast agent application [28]. Besides, it enables real-time visualization of the targeted volume as well as apparent grayscale changes during the treatment for monitoring the response to HIFU, demonstrated also in other treatment indications [20, 29].

Currently, the accepted definitive treatment of FA is breast-conserving surgery which removes the FA entirely with a subsequent tissue volume defect and risk of complications as bleeding, seroma formation and chronic incisional pain [30]. The minimally invasive US-guided vacuum-assisted percutaneous excision has better cosmetic results but incomplete removal with 3.4% to 30% rate of residual tumour associated with initial tumour size over 20 mm [13, 31]. HIFU as an ablative treatment modality competes with up-to-date established ablation methods such as radiofrequency, laser or cryoablation. However, the US-guided HIFU is entirely non-invasive, without need of probe or antenna insertion in the FA. It requires less recovery time and cost, and is associated with only minor adverse events and with no complications as hematoma, infection or scar formation [21, 26]. It can be performed in case of multiple lesions, in one or both breasts. During the long-term follow-up of our patients, the residual lesions did not demonstrate a potential for regrowth, which cannot be stated for still viable residual tissue after vacuum-assisted percutaneous excision [31]. When the results are not satisfactory after one treatment or the FA is still large, second HIFU is a good option as shown in our study.

An important drawback of the HIFU treatment is the long duration, which increases with the size of treated FA. Another limitation is one layer treatment, which may result in incomplete ablation. However, the later presumption is not supported by our observation of proportionate reduction of all three FA diameters, but larger studies are needed to evaluate the exact mechanism of FA shrinkage after ablation. Besides, an appropriate patient selection is needed in order to assure the accessibility of the targeted FA. Whereas deeply situated lesion may not be accessible to US beam, the superficial FA localization associated with scarcity of subcutaneous fat may result in skin burn as observed in one of our patients with low BMI. Certainly, the main limitation of the study is the small number of observed patients, especially in the group treated with two sessions, which is mainly due to the recent introduction of the method and lack of previous experience.


Our results of US-guided HIFU ablation of breast FA provide evidence that the method is promising, efficient and safe. Although one treatment session results in marked reduction of FA volume, the second treatment significantly increases the degree of volume reduction. Due to its non-invasive nature, the method is well tolerated and associated with high level of patients’ satisfaction. Therefore, US-guided HIFU may become an alternative to other well-established treatment modalities of breast FA. However, larger cohorts of patients with similar or even longer follow-up should be evaluated in order to define the treatment success.



Breast imaging and reporting data system


Body mass index


Colour flow Doppler




High-intensity focused ultrasound


Magnetic resonance


Standard deviation




Visual analog scale.


  1. Olu-Eddo AN, Ugiagbe EE. Benign breast lesions in an African population: A 25-year histopathological review of 1864 cases. Niger Med J. 2011;52:211–16.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Aslam HM, Saleem S, Shaikh HA, Shahid N, Mughal A, Umah R. Clinico- pathological profile of patients with breast diseases. Diagn Pathol. 2013;8:77.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Greenberg R, Skornick Y, Kaplan O. Management of breast fibroadenomas. J Gen Intern Med. 1998;13:640–45.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Masciadri N, Ferranti C. Benign breast lesions: Ultrasound. J Ultrasound. 2011;14:55–65.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Guray M, Sahin AA. Benign breast diseases: classification, diagnosis, and management. Oncologist. 2006;11:435–49.

    Article  PubMed  Google Scholar 

  6. Santen RJ, Mansel R. Benign breast disorders. N Engl J Med. 2005;353:275–85.

    Article  CAS  PubMed  Google Scholar 

  7. Dixon JM, Dobie V, Lamb J, Walsh JS, Chetty U. Assessment of the acceptability of conservative management of fibroadenoma of the breast. Br J Surg. 1996;83:264–65.

    Article  CAS  PubMed  Google Scholar 

  8. Carter BA, Page DL, Schuyler P, Parl FF, Simpson JF, Jensen RA, et al. No elevation in long-term breast carcinoma risk for women with fibroadenomas that contain atypical hyperplasia. Cancer. 2001;92:30–6.

    Article  CAS  PubMed  Google Scholar 

  9. Worsham MJ, Raju U, Lu M, Kapke A, Botttrell A, Cheng J, et al. Risk factors for breast cancer from benign disease in a diverse population. Breast Cancer Res Treat. 2009;118:1–7.

    Article  PubMed  Google Scholar 

  10. Sklair-Levy M, Sella T, Alweiss T, Craciun I, Libson E, Mally B. Incidence and management of complex fibroadenomas. AJR Am J Roentgenol. 2008;190:214–18.

    Article  PubMed  Google Scholar 

  11. Harvey JA, Nicholson BT, Lorusso AP, Cohen MA, Bovbjerg VE. Short-term follow-up of palpable breast lesions with benign imaging features: evaluation of 375 lesions in 320 women. AJR Am J Roentgenol. 2009;193:1723–30.

    Article  PubMed  Google Scholar 

  12. Anderson BO, Masetti R, Silverstein MJ. Oncoplastic approaches to partial mastectomy: an overview of volume displacement techniques. Lancet Oncol. 2005;6:145–57.

    Article  PubMed  Google Scholar 

  13. Grady I, Gorsuch H, Wilburn-Bailey S. Long-term outcome of benign fibroadenomas treated by ultrasound-guided percutaneous excision. Breast J. 2008;14:275–78.

    Article  PubMed  Google Scholar 

  14. Thurley P, Evans A, Hamilton L, James J, Wilson R. Patient satisfaction and efficacy of vacuum-assisted excision biopsy of fibroadenomas. Clin Radiol. 2009;64:381–85.

    Article  CAS  PubMed  Google Scholar 

  15. Hahn M, Pavlista D, Danes J, Klein R, Golatta M, Harcos A, et al. Ultrasound guided cryoablation of fibroadenomas. Ultraschall Med. 2013;34:64–8.

    CAS  PubMed  Google Scholar 

  16. Lai LM, Hall-Craggs MA, Mumtaz H, Ripley PM, Davidson TI, Kissin MW, et al. Interstitial laser photocoagulation for fibroadenomas of the breast. Breast. 1999;8:89–94.

    Article  Google Scholar 

  17. Fornage BD, Sneige N, Ross MI, Mirza AN, Kuerer HM, Edeiken BS, et al. Small (< or = 2-cm) breast cancer treated with US-guided radiofrequency ablation: feasibility study. Radiology. 2004;231:215–24.

    Article  PubMed  Google Scholar 

  18. Vargas HI, Dooley WC, Gardner RA, Gonzales KD, Venegas R, Heywang-Kobrunner SH, et al. Focused microwave phased array thermotherapy for ablation of early-stage breast cancer: results of thermal dose escalation. Ann Surg Oncol. 2004;11:139–46.

    Article  PubMed  Google Scholar 

  19. Zhou W, Zha X, Liu X, Ding Q, Chen L, Ni Y, et al. US-guided percutaneous microwave coagulation of small breast cancers: a clinical study. Radiology. 2012;263:364–73.

    Article  PubMed  Google Scholar 

  20. Zhou YF. High intensity focused ultrasound in clinical tumor ablation. World J Clin Oncol. 2011;2:8–27.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Hynynen K, Pomeroy O, Smith DN, Huber PE, McDannold NJ, Kettenbach J, et al. MR imaging–guided focused ultrasound surgery of fibroadenomas in the breast: a feasibility study. Radiology. 2001;219:176–85.

    Article  CAS  PubMed  Google Scholar 

  22. Peek MCL, Ahmed M, Scudder J, Baker R, Pinder SE, Douek M, et al. High intensity focused ultrasound in the treatment of breast fibroadenomata: results of the HIFU-F trial. Int J Hyperthermia. 2016;32:881–8.

    Article  CAS  PubMed  Google Scholar 

  23. Kovatcheva R, Guglielmina JN, Abehsera M, Boulanger L, Laurent N, Poncelet E. Ultrasound-guided high-intensity focused ultrasound treatment of breast fibroadenoma-a multicenter experience. J Ther Ultrasound. 2015;3:1.

    Article  PubMed  PubMed Central  Google Scholar 

  24. Vitti P, Rago T, Mazzeo S, Brogioni S, Lampis M, De Liperi A, et al. Thyroid blood flow evaluation by color-flow Doppler sonography distinguishes Graves' disease from Hashimoto's thyroiditis. J Endocrinol Invest. 1995;18:857–61.

    Article  CAS  PubMed  Google Scholar 

  25. Rabkin BA, Zderic V, Crum LA, Vaezy S. Biological and physical mechanisms of HIFU-induced hyperecho in ultrasound images. Ultrasound Med Biol. 2006;32:1721–9.

    Article  PubMed  Google Scholar 

  26. Furusawa H, Namba K, Thomsen S, Akiyama F, Bendet A, Tanaka C, et al. Magnetic resonance-guided focused ultrasound surgery of breast cancer: reliability and effectiveness. J Am Coll Surg. 2006;203:54–63.

    Article  PubMed  Google Scholar 

  27. Merckel LG, Knuttel FM, Deckers R, van Dalen T, Shubert G, Peters NH, et al. First clinical experience with a dedicated MRI-guided high-intensity focused ultrasound system for breast cancer ablation. Eur Radiol. 2016;26:4037–46.

    Article  PubMed  PubMed Central  Google Scholar 

  28. Marincola BC, Pediconi F, Anzidei M, Miglio E, Di Mare L, Telesca M, et al. High-intensity focused ultrasound in breast pathology: non-invasive treatment of benign and malignant lesions. Expert Rev Med Devices. 2015;12:191–9.

    Article  Google Scholar 

  29. Orsi F, Arnone P, Chen W, Zhang L. High intensity focused ultrasound ablation: a new therapeutic option for solid tumors. J Cancer Res Ther. 2010;6:414–20.

    Article  PubMed  Google Scholar 

  30. Nyström L, Andersson I, Bjurstam N, Frisell J, Nordenskjöld B, Rutqvist LE. Long-term effects of mammography screening: updated overview of the Swedish randomized trials. Lancet. 2002;359:909–19.

    Article  PubMed  Google Scholar 

  31. Wang WJ, Wang Q, Cai QP, Zhang JQ. Ultrasonographically guided vacuum-assisted excision for multiple breast masses: non-randomized comparison with conventional open excision. J Surg Oncol. 2009;100:675–80.

    Article  PubMed  Google Scholar 

Download references


The authors thank Nina Dilova, MD, for her help with patient recruitment; Albena Fakirova, MD, and Radka Hristoskova, MD, from the Pathology department of the Military Medical Academy, Sofia, Bulgaria, for analysing the speciments obtained with core needle biopsy of breast lumps.


The research was supported by Theraclion SAS (Paris, France). The sponsor defined the technical parameters of the treatment in the study design. The collection, analysis and interpretation of data and the writing of the manuscript were conducted by the authors and were not supported by the funding body.

Availability of data and materials

The datasets obtained during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Authors’ contributions

RK, KZ, JV and JS performed the US-guided HIFU treatments, data collection, data analysis, and contributed to the design and writing of the manuscript. All the authors read and approved the final manuscript.

Competing interests

The authors declare that they have no competing interests.

Consent for publication

A written informed consent to publish was obtained from all participants.

Ethics approval and consent to participate

The Ethical Committee at the University Hospital of Endocrinology of Sofia approved the study on the 8th of March 2011, and all participants signed the consent forms.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Roussanka Kovatcheva.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kovatcheva, R., Zaletel, K., Vlahov, J. et al. Long-term efficacy of ultrasound-guided high-intensity focused ultrasound treatment of breast fibroadenoma. J Ther Ultrasound 5, 1 (2017).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: