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Blood-brain barrier opening-based local delivery of 80 nm-sized liposomes in mice using pulsed focused ultrasound
© Kovacs et al; licensee BioMed Central Ltd. 2015
Published: 30 June 2015
Continuous focused ultrasound (FUS) has been mostly used to generate high temperatures necessary for tissue ablation. Pulsed FUS in combination with intravenously injected microbubbles has been shown to open the blood-brain barrier (BBB) to increase plasma-to-tissue permeability, thus presenting a new opportunity for local drug delivery to the targeted part of the brain. We have previously demonstrated a local delivery of doxorubicin to mouse brain tumor tissues by transient BBB opening. However, the next generation of therapeutics is associated with a broader range of clinically meaningful applications. Liposomes are particularly interesting since drug and contrast agent can be combined in a single safe and biodegradable system with a sustained release of content. Therefore, we investigated the brain delivery of fluorescently labeled liposomes by FUS-mediated BBB opening in healthy mice.
The 80 nm-sized vesicles were either labeled with cholesteryl 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoate (chol-BODIPY®) incorporated into the lipid bilayer or both with BODIPY® and an additional dye, whose crosstalking with BODIPY® was ruled out (IRDye® 680LT and ROX™) and was conjugated to the liposome surface. The systemically injected liposomes (100 μL) were washed out of the bloodstream 2 h after sonication. FUS was created by a single-element, spherical FUS transducer (center frequency: 612.5 kHz; focal depth: 50 mm; active diameter: 64 mm; model: H-107_MR, Sonic Concepts, Bothell, WA). All FUS sonications started 30 s after the injection of 60 μL lipid-coated gas microbubbles via the tail vein in B6 (Cg)-Tyrc-2J/J mice (n=8).
Results and conclusions
Funded by CoMe and Sinergia.
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