Fig. 5

Representative flow cytometry dot plots used to quantify sonoporation efficiency. Cells were classified as sonoporated if they showed calcein fluorescence (viability) and uptake propidium iodide (membrane permeability). These cells appear in quadrant 2 (Q2) in the dot plots. a The small percentage of cells that appear in Q2 for the sham treatment group was defined as the false positive rate and was subtracted from the percent of cells in Q2 for all treatment groups. b Ultrasound exposure below the PCCA activation threshold did not result in sonoporation. c, d Sonoporation is observed above the PCCA activation threshold and increases with increasing pressure. a Sham Control b 125 kPa - 20 cycles c 300 kPa - 20 cycles d 600 kPa - 20 cycles