Validation with the animal model. Evans blue dye leakage served asprimary validation. A.1/A.2 shows BBB opening on therat/mouse separately. A photo of the entire brain is referred at the corner;bar is 5 mm. T1‒weighted spin‒echomulti‒slice MR images (TR/TE, 151/10 ms) were acquired with ratas secondary validation. B.1 and B.2 show images intransverse and coronal directions, respectively. Yellow rectanglehighlighted the brain, and contrast enhancement (white arrow)indicates localized BBB opening.