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Fig. 4 | Journal of Therapeutic Ultrasound

Fig. 4

From: Transcranial and pulsed focused ultrasound that activates brain can accelerate remyelination in a mouse model of multiple sclerosis

Fig. 4

Analysis method of histology images of myelin. a Original color brightfield image. Note that the left portion of these brain images correspond to the left hemisphere of the mouse brain. b Color thresholding (shown in red) on the same image. Thresholding was done in color since saturation for myelinated tissues showed variation relative to unmyelinated regions. c Resulting black and white mask derived from (b). d Black and white mask with grid and both regions of interest outlined in red. Note that the left outline does not extend past the second tissue fold (anomaly), and that the length of the right outline matches that of the left, by design. e 8-bit black and white original image with both ROIs overlaid in red. We quantify the grey values indicative of myelin from this image, with darker hence more grey pixels associated with more myelin

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