Fig. 1 (abstract O52).

Experimental arrangement used. A gel sandwich consisting of a thin (~300μm thick), cell containing collagen gel and two 6mm thick slices of PVA cryo-gel is enclosed in a sterile sample holder filled with degassed cell culture medium. Top and bottom of the sample holder were sealed with tight Mylar membranes to minimize beam reflection. The sample holder is placed with the cell layer located at the focal plane of a focused single element transducer in a water tank. For treatments, the transducer is moved on a circular trajectory with temperature being monitored by a fine wire thermocouple at the center of the circle at the cell layer