Fig. 1

a A typical example of shot planning of two clusters of seven treatment cells sonicated under general anesthesia (GA) and procedural sedation and analgesia (PSA) in the liver (L). GB gall bladder. b A typical example of heating in the focal point under GA in the sagittal plane., whereby the color scale ranges from 43 °C (blue) to 70 °C (red). c A typical example of heating under PSA in the sagittal plane using the same color bar as b. d Example of nicotinamide adenine dinucleotide (NADH) staining of a lesion ablated under general anesthesia or under procedural sedation and analgesia e. Clearly distinguishable regions containing non-viable cells are recognized by its lack of color, while viable cells turn blue after staining