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Figure 5 | Journal of Therapeutic Ultrasound

Figure 5

From: In vitro methods for evaluating therapeutic ultrasound exposures: present-day models and future innovations

Figure 5

Chitosan-gelatin biological scaffolds. (left) 2D scaffold: (a) brightfield image showing the fibrous structure of the scaffold; (b) fluorescent image of the same scaffold in (a), where the nuclei of fibroblasts are visible, stained with DAPI. Bar = 100 μm. (right) 3D scaffolds sectioned, stained with Masson's trichrome (red, scaffold; purple, fibroblasts), and observed with brightfield microscopy. (c) Edge region of a non-cellularized scaffold (bar = 200 μm). (inset) Entire scaffold (height = 7 mm; radius = 20 mm). (d,e) Regions of cellularized scaffolds (outer surface at top) (bar = 50 μm). Pore sizes range from 50 to 200 μm, with various degrees of cellularization.

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