Focused ultrasound-mediated drug delivery to pancreatic cancer in a mouse model

Table 1 Treatment parameters for all animals used in the study

N	Treatment group	MRgHIFU parameters				Time between injection and MRgFUS (h)	Life span (weeks)^a
N	Treatment group	Trajectory	Acoustic pressure (MPa)	Duty cycle (%), pulse length	Total sonication time (s)	Time between injection and MRgFUS (h)	Life span (weeks)^a
7	Negative control (no injection, no MRgFUS)	N/A	N/A	N/A	N/A	N/A	3.5 ± 0.5
7	PTX-nanodroplets + No MRgFUS	N/A	N/A	N/A	N/A		7.0 ± 0.8
1	No injection + MRgFUS	Spiral	3.4	100	240		4.8 ± 2.3
1		Spiral	4.2		60
4		Grid	3.4		325 ± 29
4		Grid	4.2		300
3	Empty nanodroplets + No MRgFUS	N/A	N/A	N/A	N/A	6 to 8	3.5 ± 0.5
1	Empty nanodroplets + MRgFUS	Spiral	3.4	100	60	6 to 8	3.5 ± 2.1^b
2			4.2		60
1			4.8		240
4		Grid	3.4		313 ± 25
4	PTX-nanodroplets + MRgFUS	Grid	3.4	100	300	1 to 2	7.0 ± 1.0
1		Grid	2.4	100	300	1 to 2	7.0 ± 1.0
4		Spiral	3.4	100	153 ± 54	6 to 8	10.3 ± 1.6^c
4			2.4	100	130 ± 50
1			4.2	100	60
1			4.8	100	60
5		Grid	3.4	100	300
2			4.8	100	300
2			4.2	100	300
1		Grid	3.4	50 (50 ms)	300		6.0 ± 1.4
1		Grid	4.2	50 (50 ms)	300
1		Spiral	3.4	50 (100 ms)	300
1		Spiral	4.8	50 (50 ms)	300

^aMean values plus/minus standard deviations are presented; ^bMice that died within several days after the treatment (observed for the MRgFUS pressure of or higher than 4.2 MPa) were excluded from the life span calculation; ^cSurvivors (N = 2 for the grid trajectory) were excluded from the life span calculation.

ISSN: 2050-5736