Fig. 2

Global view of the experiment. a Plan view. We delivered ultrasound transcutaneously and transcranially to the left side of mouse brain, using a micro-positioner to deliver that ultrasound to − 1.03 mm behind Bregma, 0.5 mm to the right of center, and 0.5 mm below the skull – within the corpus callosum, below the somatosensory cortex and above the hippocampus. We placed two subdermal electrodes on either side of the ultrasound focus. We placed a reference electrode rostral to the site of ultrasound application, and a grounding electrode subcutaneously on the mouse’s shoulder. b Coronal view. This schematic shows a coronal view of mouse brain, stained for myelin, at − 1.03 mm Bregma, highlighting the corpus callosum (the ‘eye brow-like’ black and curved line), the hippocampus (HC) and the point of ultrasound exposure