Fig. 1 (abstract O43).

Trans­membrane drug delivery under flow condition. Human umbilical vein endothelial cell (HUVEC) were stained for cell viability by calcein­AM (green cells) and trans­membrane delivery by internalization of propidium iodide (red cells). (a) Microbubbles were made to flow (fluid velocity: 95 mm/s) through a collagen­treatedflow chamber with a channel height of 600 μm and a volume of 150 μL. (b) The regioin (dotted rectangle) acted as non­sonicated control region, while (c) central chamber region acted as sonicated region (solid rectangle). (d) Zoomed in view at delivery region; a population of cell that exhibited a mixture of viable cells (green cells) with and without trans­membrane delivery and dead cell (red cell)